Abstract
In this study, a sensitive and reliable method for the quantitation of SK3497 in rat
plasma was developed and validated using high performance liquid chromatography. The
plasma samples were prepared by deproteinization, and sildenafil was used as an internal
standard. Chromatographic separation was achieved using a reversed-phase (C18) column.
The mobile phase, 0.02 M ammonium acetate buffer:acetonitrile (45:55, v/v), was run
at a flow rate of 1.0 mL/min, and the column eluent was monitored using an ultraviolet
detector at 254 nm at room temperature. The retention times of sildenafil (an internal
standard), and SK3497 were approximately 5.6 and 8.3 min, respectively. The detection
limit of SK3497 in rat plasma was 0.03 μg/mL. Pharmacokinetic parameters of SK3497
was evaluated after intravenous (i. v.; at doses of 15 mg/kg) and oral (p.o.; at doses
of 30 mg/kg) administration of SK3497 in rats. After p.o. administration (30 mg/kg)
of SK3497, F-value was approximately 53.0%. The protein binding of SK3497 to 4% human serum albumin
were also described.
Key words
SK3497 - sildenafil - bioavailability - pharmacokinetics - rats
